X-ray-scattering of turkey skeletal-muscle troponin C in solution at low pH.
نویسندگان
چکیده
The solution structure of troponin C from turkey skeletal muscle was studied at low pH by small-angle X-ray-scattering. We find that troponin C at pH 5.3 in the presence of Mg2+ has a triaxial radius of gyration and maximum dimension comparable with those of the crystallized protein. However, the relative disposition of domains is more similar to that found for the highly homologous rabbit protein in solution at pH 7.4.
منابع مشابه
Small-angle x-ray scattering investigation of the solution structure of troponin C.
X-ray crystallographic studies of troponin C (Herzberg, O., and James, M.N.G. (1985) Nature 313, 653-659; Sundaralingam, M., Bergstrom, R., Strasburg, G., Rao, S.T., and Roychowdhury, P. (1985a) Science 227, 945-948) have revealed a novel protein structure consisting of two globular domains, each containing two Ca2+-binding sites, connected via a nine-turn alpha-helix, three turns of which are ...
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 261 3 شماره
صفحات -
تاریخ انتشار 1989